Enjoyed the Freaky Five discussion on killing off characters, and, though I'm a day late and a dollar short, I do feel compelled to give my own list ...
First, though, a few general thoughts on killing off major characters. Basically, I figure if you are a writer and you kill off a major character, you are on a major hiding to nothing because it's inevitable that they're going to be brought back sooner or later.
To illustrate, let's look at the track record of one Jean-Marc DeMatteis. I bow to no one in my love of DeMatteis' writing, but it has to be said that the "Touching Death of a Major Character" story has become something of a standard schtick for him over the years. In the mid-90s at Marvel, for example, DeMatteis went on a bit of a rampage, killing off characters at the pace of one a year. In 1993, it was Harry Osborn (
Spectacular Spider-Man #200); in 1994 he did in Aunt May (
Amazing Spider-Man #400); and in 1995 he offed Jamie Madrox (
X-Factor #100). All of these characters were, of course, subsequently resurrected.
And, lest we forget, this is the same J M DeMatteis who offed the Red Skull in 1984 (
Captain America #300) and Kraven the Hunter in 1987 (
Amazing Spider-Man #294). Both of whom were, of course, subsequently resurrected. In fact '87 was another big year for DeMatteis, because it was also the year that he knocked off the original Doctor Fate, Kent Nelson, in the
Doctor Fate mini series. Nelson was, of course ... well, you get the picture.
Anyway, the point is that it's pretty easy to off a character for dramatic effect or pathos, but when you are working in an industry where your story it's inevitable your story is eventually going to be undone or retconned, maybe you're better off to make a bit more effort and come up with an interesting story that doesn't rely on that crutch ...?
With all that having been said, let's kill off some characters!

Here's my top 5 characters who should be killed off and never, ever brought back:
5: Norman Osborn - his resurrection undermined a truly classic story, and when they brought him back they turned him into a rip-off of the Man of Steel-era Lex Luthor (which is nothing like how Norman had previously been depicted).
4: Jason Todd - for all the reasons discussed in the podcast.
3: Bucky - sorry, folks, but there are some rules that should never be broken and one of them is "only Bucky and Uncle Ben stay dead." Plus, bringing him back as a cyborg assassin is a horrendous plot device that should have stayed in the '90s where it belongs (didn't they do the same trick with Moon Knight's sidekick back then?).
2: Psylocke - a character so utterly ruined by retcon upon retcon that the only thing left to do with her is put her out of her misery.
1: Gambit - anyone remember when Gambit was cool? Basically, Marvel has had no idea what to do with this guy since Jim Lee left the X-Men about 20 years ago. Plus, that accent is REALLY annoying.
Bubbling under: Hal Jordan, Barry Allen, Gwen Stacey's kids from "Sins Past".
R